Laboratory Tour & Capabilities

Our laboratory is equipped with twelve (12) hoods for organic synthesis, each with a dual-manifold Schlenk line, compressed air / vacuum, and plenty of stir plates! The laboratory is also fitted with one hood specifically dedicated for photochemistry (housing our photoreactor) and another for ozonolysis (fitted with our ozone generator).  Our hoods are spread across three laboratory bays in the ASDRP Chemistry & Chemical Biology laboratory (Bay 1 Discovery Chemistry, Bay 2 Discovery Chemistry, Bay 3 Process Chemistry). Our synthetic labs are fully equipped with dessicators, bench space, and ten (10) Buchi rotary evaporators (affectionately referred to as "rotavaps").

Nuclear magnetic resonance (NMR) spectroscopy is the heart and soul of characterization in organic synthesis. In the ASDRP chemistry building core spectroscopy building, we are privileged to operate six benchtop NMR spectrometers from Nanalysis (3 x NMReady 60PRO QUAD) and Thermo (1 x PicoSpin 80 Series II, 2 x Picospin 45). Things we are capable of:

• Standard 1H, 13C, 19F, 31P 1D NMR spectra

• J-Resolved (JRES) spectroscopy for resolving overlapping resonances

• Flow chemistry with in-line NMR

• COSY, HSQC, HMBC, HETCOR 2D NMR for complex structural elucidation

• T1/T2 or Hahn Echo for relaxometry experiments

Together with the Yamamoto laboratory, the Njoo lab shares occupancy of the ASDRP Chemistry Building Core mass spectrometry facility, which includes nine mass spectrometers:

• Two Thermo Trace / DSQ single quadrupole GC-MS instruments

• One Thermo LTQ-XL linear ion trap LC-MS

• One Thermo TSQ triple quadrupole LC-MS

• One Waters Micromass Quattro triple quadrupole LC-MS

• One Waters SQD single quadrupole LC-MS

• One Advion ExpressION direct injection ASAP single quad MS

• One Waters Xevo Q-TOF MS

• One Waters LCT microTOF LC-MS

• In addition, we share several high performance liquid chromatography (HPLC) instruments with the Yamamoto lab.

We share two cell culture laboratories with our colleagues in the Chemistry Department at ASDRP, with a total of three cell culture hoods, three CO2 incubators, and four flow cytometers, including three BD C6 Accuri analytical flow cytometers and one BD FACS for single cell sorting. In addition, we use ultralow freezers (-80 degree freezers) for mammalian cell culture.

In our shared process chemistry and instrumentation lab, we operate the following instrumentation:

• Waters Dawn Helios Multi-Angle Light Scattering SEC-MALS system

• Waters DynaPro high throughput dynamic light scattering (DLS)

• Malvern Analytical Zeta Sizer

• Dynamic Scanning Calorimetry

• Surface Plasmon Resonance (SPR)

• CSBio solid phase peptide synthesizer

The Njoo lab houses, in our shared core spectroscopy facility:

• Thermo iS5 Fourier transform infrared (FT-IR) spectrometer

• Perkin Elmer Lambda 11 UV-vis spectrophotometer

• Two Tecan fluorescence and bioluminescence plate readers

• Thunder Optics RAMAN spectrometer

• Rudolph Autopol Polarimeter

• Cyclic voltammetry for redox measurements

Our laboratory's biochemistry instrumentation includes:

• AKTA protein purification system

• Two Nanodrop micro UV-vis spectrophotometers

• Two Bio-Rad quantitative PCR (qPCR) instruments

• One Applied Biosystems qPCR instrument

• Thermo gel imager

• Bio-Rad electroporation apparatus

• Electrophoresis station for gel electrophoresis and SDS-PAGE